Insertion of disease-causing mutations in BACs by homologous recombination in Escherichia coli

We have used GET Recombination, an inducible homologous recombination system for Escherichia coli, to insert one of the most common thalassaemia mutations into the intact β-globin locus in a second generation BAC vector. We first inserted a PCR fragment carrying the tetracycline resistance gene (Tet...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Nefedov, Mikhail, Williamson, Robert, Ioannou, Panayiotis A.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2000
Soggetti:	NAR Methods Online
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC110716/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10954612/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.17.e79
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