Insertion of disease-causing mutations in BACs by homologous recombination in Escherichia coli
We have used GET Recombination, an inducible homologous recombination system for Escherichia coli, to insert one of the most common thalassaemia mutations into the intact β-globin locus in a second generation BAC vector. We first inserted a PCR fragment carrying the tetracycline resistance gene (Tet...
Αποθηκεύτηκε σε:
| Εκδόθηκε σε: | Nucleic Acids Res |
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| Κύριοι συγγραφείς: | , , |
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
Oxford University Press
2000
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| Θέματα: | |
| Διαθέσιμο Online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC110716/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10954612/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.17.e79 |
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