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Rare Homologous Gene Targeting in Histoplasma capsulatum: Disruption of the URA5(Hc) Gene by Allelic Replacement

URA5 genes encode orotidine-5′-monophosphate pyrophosphorylase (OMPpase), an enzyme involved in pyrimidine biosynthesis. We cloned the Histoplasma capsulatum URA5 gene (URA5(Hc)) by using a probe generated by PCR with inosine-rich primers based on relatively conserved sequences in OMPpases from othe...

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Udgivet i:J Bacteriol
Principais autores: Woods, Jon P., Retallack, Diane M., Heinecke, Elizabeth L., Goldman, William E.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1998
Fag:
Eukaryotic Cells
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC107550/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9748447/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.180.19.5135-5143.1998
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