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Rare Homologous Gene Targeting in Histoplasma capsulatum: Disruption of the URA5(Hc) Gene by Allelic Replacement

URA5 genes encode orotidine-5′-monophosphate pyrophosphorylase (OMPpase), an enzyme involved in pyrimidine biosynthesis. We cloned the Histoplasma capsulatum URA5 gene (URA5(Hc)) by using a probe generated by PCR with inosine-rich primers based on relatively conserved sequences in OMPpases from othe...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Woods, Jon P., Retallack, Diane M., Heinecke, Elizabeth L., Goldman, William E.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology 1998
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC107550/
https://ncbi.nlm.nih.gov/pubmed/9748447
Tagiau: Ychwanegu Tag
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