Rare Homologous Gene Targeting in Histoplasma capsulatum: Disruption of the URA5(Hc) Gene by Allelic Replacement

URA5 genes encode orotidine-5′-monophosphate pyrophosphorylase (OMPpase), an enzyme involved in pyrimidine biosynthesis. We cloned the Histoplasma capsulatum URA5 gene (URA5(Hc)) by using a probe generated by PCR with inosine-rich primers based on relatively conserved sequences in OMPpases from othe...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Woods, Jon P., Retallack, Diane M., Heinecke, Elizabeth L., Goldman, William E.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1998
Schlagworte:	Eukaryotic Cells
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC107550/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9748447/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.180.19.5135-5143.1998
Tags:	Tag hinzufügen Keine Tags, Fügen Sie das erste Tag hinzu!