Poly(A) Polymerase Modification and Reverse Transcriptase PCR Amplification of Environmental RNA

We describe a combination of two established techniques for a novel application for constructing full-length cDNA clone libraries from environmental RNA. The cDNA was cloned without the use of prescribed primers that target specific genes, and the procedure did not involve random priming. Purified R...

पूर्ण विवरण

में बचाया:
ग्रंथसूची विवरण
मुख्य लेखकों: Botero, Lina M., D'Imperio, Seth, Burr, Mark, McDermott, Timothy R., Young, Mark, Hassett, Daniel J.
स्वरूप: Artigo
भाषा:Inglês
प्रकाशित: American Society for Microbiology 2005
विषय:
Methods
ऑनलाइन पहुंच:https://ncbi.nlm.nih.gov/pmc/articles/PMC1065135/
https://ncbi.nlm.nih.gov/pubmed/15746328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.3.1267-1275.2005
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