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Poly(A) Polymerase Modification and Reverse Transcriptase PCR Amplification of Environmental RNA

We describe a combination of two established techniques for a novel application for constructing full-length cDNA clone libraries from environmental RNA. The cDNA was cloned without the use of prescribed primers that target specific genes, and the procedure did not involve random priming. Purified R...

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Bibliografski detalji
Glavni autori: Botero, Lina M., D'Imperio, Seth, Burr, Mark, McDermott, Timothy R., Young, Mark, Hassett, Daniel J.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2005
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1065135/
https://ncbi.nlm.nih.gov/pubmed/15746328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.3.1267-1275.2005
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