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Poly(A) Polymerase Modification and Reverse Transcriptase PCR Amplification of Environmental RNA
We describe a combination of two established techniques for a novel application for constructing full-length cDNA clone libraries from environmental RNA. The cDNA was cloned without the use of prescribed primers that target specific genes, and the procedure did not involve random priming. Purified R...
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| Autori principali: | , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Microbiology
2005
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1065135/ https://ncbi.nlm.nih.gov/pubmed/15746328 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.3.1267-1275.2005 |
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