Reverse Transcriptase PCR Amplification of Rickettsia typhi from Infected Mammalian Cells and Insect Vectors

We developed a reverse transcriptase PCR assay to detect expression of 120- and 17-kDa antigen genes in Rickettsia typhi. Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers. The cDNA was amplified by using high concentrations of primer and template in an inexpensive,...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Higgins, James A., Radulovic, Suzana, Noden, Bruce H., Troyer, Jill M., Azad, Abdu F.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Bacteriology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC104925/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9620425/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.36.6.1793-1794.1998
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