Poly(A) Polymerase Modification and Reverse Transcriptase PCR Amplification of Environmental RNA

We describe a combination of two established techniques for a novel application for constructing full-length cDNA clone libraries from environmental RNA. The cDNA was cloned without the use of prescribed primers that target specific genes, and the procedure did not involve random priming. Purified R...

詳細記述

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書誌詳細
主要な著者: Botero, Lina M., D'Imperio, Seth, Burr, Mark, McDermott, Timothy R., Young, Mark, Hassett, Daniel J.
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology 2005
主題:
Methods
オンライン･アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1065135/
https://ncbi.nlm.nih.gov/pubmed/15746328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.3.1267-1275.2005
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