Poly(A) Polymerase Modification and Reverse Transcriptase PCR Amplification of Environmental RNA

We describe a combination of two established techniques for a novel application for constructing full-length cDNA clone libraries from environmental RNA. The cDNA was cloned without the use of prescribed primers that target specific genes, and the procedure did not involve random priming. Purified R...

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Detalhes bibliográficos
Main Authors: Botero, Lina M., D'Imperio, Seth, Burr, Mark, McDermott, Timothy R., Young, Mark, Hassett, Daniel J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1065135/
https://ncbi.nlm.nih.gov/pubmed/15746328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.3.1267-1275.2005
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