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Characterization of an Extremely Thermostable Restriction Enzyme, PspGI, from a Pyrococcus Strain and Cloning of the PspGI Restriction-Modification System in Escherichia coli

An extremely thermostable restriction endonuclease, PspGI, was purified from Pyrococcus sp. strain GI-H. PspGI is an isoschizomer of EcoRII and cleaves DNA before the first C in the sequence 5′ ^CCWGG 3′ (W is A or T). PspGI digestion can be carried out at 65 to 85°C. To express PspGI at high levels...

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Autors principals: Morgan, Richard, Xiao, Jian-ping, Xu, Shuang-yong
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 1998
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC106503/
https://ncbi.nlm.nih.gov/pubmed/9758783
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