Characterization of a Highly Thermostable Alkaline Phosphatase from the Euryarchaeon Pyrococcus abyssi

This work reports the first isolation and characterization of an alkaline phosphatase (AP) from a hyperthermophilic archaeon. An AP gene from Pyrococcus abyssi, a euryarchaeon isolated from a deep-sea hydrothermal vent, was cloned and the enzyme expressed in Escherichia coli. Analysis of the sequenc...

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Bibliografiske detaljer
Main Authors: Zappa, Sébastien, Rolland, Jean-Luc, Flament, Didier, Gueguen, Yannick, Boudrant, Joseph, Dietrich, Jacques
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2001
Fag:
Enzymology and Protein Engineering
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC93196/
https://ncbi.nlm.nih.gov/pubmed/11571149
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.10.4504-4511.2001
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