Construction of a Shuttle Vector for, and Spheroplast Transformation of, the Hyperthermophilic Archaeon Pyrococcus abyssi

Our understanding of the genetics of species of the best-studied hyperthermophilic archaea, Pyrococcus spp., is presently limited by the lack of suitable genetic tools, such as a stable cloning vector and the ability to select individual transformants on plates. Here we describe the development of a...

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Autori principali: Lucas, Soizick, Toffin, Laurent, Zivanovic, Yvan, Charlier, Daniel, Moussard, Hélène, Forterre, Patrick, Prieur, Daniel, Erauso, Gaël
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2002
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Methods
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC129897/
https://ncbi.nlm.nih.gov/pubmed/12406746
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.11.5528-5536.2002
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