Construction of a Shuttle Vector for, and Spheroplast Transformation of, the Hyperthermophilic Archaeon Pyrococcus abyssi

Our understanding of the genetics of species of the best-studied hyperthermophilic archaea, Pyrococcus spp., is presently limited by the lack of suitable genetic tools, such as a stable cloning vector and the ability to select individual transformants on plates. Here we describe the development of a...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Lucas, Soizick, Toffin, Laurent, Zivanovic, Yvan, Charlier, Daniel, Moussard, Hélène, Forterre, Patrick, Prieur, Daniel, Erauso, Gaël
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2002
Assuntos:	Methods
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC129897/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12406746/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.11.5528-5536.2002
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