Characterization of a Highly Thermostable Alkaline Phosphatase from the Euryarchaeon Pyrococcus abyssi

This work reports the first isolation and characterization of an alkaline phosphatase (AP) from a hyperthermophilic archaeon. An AP gene from Pyrococcus abyssi, a euryarchaeon isolated from a deep-sea hydrothermal vent, was cloned and the enzyme expressed in Escherichia coli. Analysis of the sequenc...

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Publicado en:	Appl Environ Microbiol
Autores principales:	Zappa, Sébastien, Rolland, Jean-Luc, Flament, Didier, Gueguen, Yannick, Boudrant, Joseph, Dietrich, Jacques
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 2001
Materias:	Enzymology and Protein Engineering
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC93196/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11571149/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.67.10.4504-4511.2001
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