Genomic Analysis of Clostridium botulinum Group II by Pulsed-Field Gel Electrophoresis

Pulsed-field gel electrophoresis (PFGE) was optimized for genomic analyses of Clostridium botulinum (nonproteolytic) group II. DNA degradation problems caused by extracellular DNases were overcome by fixation of cells with formaldehyde prior to isolation. A rapid (4-h) in situ DNA isolation method w...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Hielm, Sebastian, Björkroth, Johanna, Hyytiä, Eija, Korkeala, Hannu
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Food Microbiology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106105/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9464411/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.2.703-708.1998
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