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Genomic Analysis of Clostridium botulinum Group II by Pulsed-Field Gel Electrophoresis

Pulsed-field gel electrophoresis (PFGE) was optimized for genomic analyses of Clostridium botulinum (nonproteolytic) group II. DNA degradation problems caused by extracellular DNases were overcome by fixation of cells with formaldehyde prior to isolation. A rapid (4-h) in situ DNA isolation method w...

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Bibliografische gegevens
Gepubliceerd in:Appl Environ Microbiol
Hoofdauteurs: Hielm, Sebastian, Björkroth, Johanna, Hyytiä, Eija, Korkeala, Hannu
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology (ASM) 1998
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106105/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9464411/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.2.703-708.1998
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