Comparison of Culture and PCR for Detection of Burkholderia cepacia in Sputum Samples of Patients with Cystic Fibrosis

We investigated the utility of PCR to detect Burkholderia cepacia directly in sputum samples at two cystic fibrosis (CF) centers serving children and adults. Following liquefaction of the sputa by using N-acetyl-l-cysteine, DNA was isolated and analyzed by PCRs with three different primer pairs dire...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Whitby, Paul W., Dick, Hani L. N., Campbell, Preston W., Tullis, D. Elizabeth, Matlow, Anne, Stull, Terrence L.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Bacteriology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC104893/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9620393/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.36.6.1642-1645.1998
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