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Comparison of Culture and PCR for Detection of Burkholderia cepacia in Sputum Samples of Patients with Cystic Fibrosis

We investigated the utility of PCR to detect Burkholderia cepacia directly in sputum samples at two cystic fibrosis (CF) centers serving children and adults. Following liquefaction of the sputa by using N-acetyl-l-cysteine, DNA was isolated and analyzed by PCRs with three different primer pairs dire...

詳細記述

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書誌詳細
出版年:J Clin Microbiol
主要な著者: Whitby, Paul W., Dick, Hani L. N., Campbell, Preston W., Tullis, D. Elizabeth, Matlow, Anne, Stull, Terrence L.
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology (ASM) 1998
主題:
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC104893/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9620393/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.36.6.1642-1645.1998
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