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Comparison of Culture and PCR for Detection of Burkholderia cepacia in Sputum Samples of Patients with Cystic Fibrosis

We investigated the utility of PCR to detect Burkholderia cepacia directly in sputum samples at two cystic fibrosis (CF) centers serving children and adults. Following liquefaction of the sputa by using N-acetyl-l-cysteine, DNA was isolated and analyzed by PCRs with three different primer pairs dire...

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Published in:J Clin Microbiol
Main Authors: Whitby, Paul W., Dick, Hani L. N., Campbell, Preston W., Tullis, D. Elizabeth, Matlow, Anne, Stull, Terrence L.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1998
Subjects:
Bacteriology
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC104893/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9620393/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.36.6.1642-1645.1998
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