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Comparison of Culture and PCR for Detection of Burkholderia cepacia in Sputum Samples of Patients with Cystic Fibrosis

We investigated the utility of PCR to detect Burkholderia cepacia directly in sputum samples at two cystic fibrosis (CF) centers serving children and adults. Following liquefaction of the sputa by using N-acetyl-l-cysteine, DNA was isolated and analyzed by PCRs with three different primer pairs dire...

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Bibliografski detalji
Glavni autori: Whitby, Paul W., Dick, Hani L. N., Campbell, Preston W., Tullis, D. Elizabeth, Matlow, Anne, Stull, Terrence L.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 1998
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC104893/
https://ncbi.nlm.nih.gov/pubmed/9620393
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