Early melting of supercoiled DNA topoisomers observed by TGGE
We have used temperature gradient gel electrophoresis (TGGE) to measure the progress of local denaturation in closed circular topoisomer DNA as a function of temperature and superhelicity (σ). We describe the versatility of this method as a tool for detecting various conformational modifications of...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2000
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102636/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10871350/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.11.e51 |
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