Early melting of supercoiled DNA topoisomers observed by TGGE

We have used temperature gradient gel electrophoresis (TGGE) to measure the progress of local denaturation in closed circular topoisomer DNA as a function of temperature and superhelicity (σ). We describe the versatility of this method as a tool for detecting various conformational modifications of...

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Библиографические подробности

Опубликовано в::	Nucleic Acids Res
Главные авторы:	Víglask, Viktor, Antalík, Marián, Adamèík, Jozef, Podhradsk, Duan
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Oxford University Press 2000
Предметы:	NAR Methods Online
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102636/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10871350/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.11.e51
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