An automated microplate-based method for monitoring DNA strand breaks in plasmids and bacterial artificial chromosomes

A method is described for high-throughput monitoring of DNA backbone integrity in plasmids and artificial chromosomes in solution. The method is based on the denaturation properties of double-stranded DNA in alkaline conditions and uses PicoGreen fluorochrome to monitor denaturation. In the present...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Rock, Cassandra, Shamlou, Parviz Ayazi, Levy, M. Susana
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2003
Soggetti:	NAR Methods Online
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC156742/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12771229/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng065
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