An automated microplate-based method for monitoring DNA strand breaks in plasmids and bacterial artificial chromosomes
A method is described for high-throughput monitoring of DNA backbone integrity in plasmids and artificial chromosomes in solution. The method is based on the denaturation properties of double-stranded DNA in alkaline conditions and uses PicoGreen fluorochrome to monitor denaturation. In the present...
Guardat en:
| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2003
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC156742/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12771229/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng065 |
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