Escherichia coli strains that allow antibiotic-free plasmid selection and maintenance by repressor titration
We report the construction of two novel Escherichia coli strains (DH1lacdapD and DH1lacP2dapD) that facilitate the antibiotic-free selection and stable maintenance of recombinant plasmids in complex media. They contain the essential chromosomal gene, dapD, under the control of the lac operator/promo...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC29739/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11222777/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.5.e26 |
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