Escherichia coli strains that allow antibiotic-free plasmid selection and maintenance by repressor titration

We report the construction of two novel Escherichia coli strains (DH1lacdapD and DH1lacP2dapD) that facilitate the antibiotic-free selection and stable maintenance of recombinant plasmids in complex media. They contain the essential chromosomal gene, dapD, under the control of the lac operator/promo...

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Bibliographic Details

Published in:	Nucleic Acids Res
Main Authors:	Cranenburgh, Rocky M., Hanak, Julian A. J., Williams, Steven G., Sherratt, David J.
Format:	Artigo
Language:	Inglês
Published:	Oxford University Press 2001
Subjects:	NAR Methods Online
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC29739/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11222777/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.5.e26
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