TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5′-ACGGA(N(11/9))-3′

A novel prototype class-IIS restriction endonuclease, TspGWI, was isolated from the thermophilic bacterium Thermus sp. GW. The recognition sequence and cleavage positions have been established: TspGWI recognizes the non-palindromic 5-bp sequence 5′-ACGGA-3′ and cleaves the DNA 11 and 9 nt downstream...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	üylicz-Stachula, Agnieszka, Harasimowicz-Sßowiáska, Renata I., Sobolewski, Ireneusz, Skowron, Piotr M.
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 2002
Fag:	NAR Methods Online
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC101857/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11917039/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/30.7.e33
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