Chemically-induced affinity star restriction specificity: a novel TspGWI/sinefungin endonuclease with theoretical 3-bp cleavage frequency
The type IIS/IIC restriction endonuclease TspGWI recognizes the sequence 5′-ACGGA-3′, cleaving DNA 11/9 nucleotides downstream. Here we show that sinefungin, a cofactor analog of S-adenosyl methionine, induces a unique type of relaxation in DNA recognition specificity. In the presence of sinefungin,...
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| Автори: | , , , |
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| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Taylor & Francis Group
2011-06-01
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| Серія: | BioTechniques |
| Предмети: | |
| Онлайн доступ: | https://www.future-science.com/doi/10.2144/000113685 |
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