Chemically-induced affinity star restriction specificity: a novel TspGWI/sinefungin endonuclease with theoretical 3-bp cleavage frequency

The type IIS/IIC restriction endonuclease TspGWI recognizes the sequence 5′-ACGGA-3′, cleaving DNA 11/9 nucleotides downstream. Here we show that sinefungin, a cofactor analog of S-adenosyl methionine, induces a unique type of relaxation in DNA recognition specificity. In the presence of sinefungin,...

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Hlavní autoři:	Agnieszka Żylicz-Stachula, Olga Żołnierkiewicz, Joanna Jeżewska-Frąckowiak, Piotr M. Skowron
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Taylor & Francis Group 2011-06-01
Edice:	BioTechniques
Témata:	New specificity cloning technique TspGWI restriction endonuclease
On-line přístup:	https://www.future-science.com/doi/10.2144/000113685
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