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TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5′-ACGGA(N(11/9))-3′

A novel prototype class-IIS restriction endonuclease, TspGWI, was isolated from the thermophilic bacterium Thermus sp. GW. The recognition sequence and cleavage positions have been established: TspGWI recognizes the non-palindromic 5-bp sequence 5′-ACGGA-3′ and cleaves the DNA 11 and 9 nt downstream...

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Bibliografiska uppgifter
I publikationen:Nucleic Acids Res
Huvudupphov: üylicz-Stachula, Agnieszka, Harasimowicz-Sßowiáska, Renata I., Sobolewski, Ireneusz, Skowron, Piotr M.
Materialtyp: Artigo
Språk:Inglês
Utgiven: Oxford University Press 2002
Ämnen:
Länkar:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC101857/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11917039/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/30.7.e33
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