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TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5′-ACGGA(N(11/9))-3′

A novel prototype class-IIS restriction endonuclease, TspGWI, was isolated from the thermophilic bacterium Thermus sp. GW. The recognition sequence and cleavage positions have been established: TspGWI recognizes the non-palindromic 5-bp sequence 5′-ACGGA-3′ and cleaves the DNA 11 and 9 nt downstream...

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Bibliografske podrobnosti
Main Authors: üylicz-Stachula, Agnieszka, Harasimowicz-Sßowiáska, Renata I., Sobolewski, Ireneusz, Skowron, Piotr M.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2002
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Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC101857/
https://ncbi.nlm.nih.gov/pubmed/11917039
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