Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability

Most studies using knockout technologies to examine protein function have relied either on shutting off transcription (conventional conditional knockouts with tetracycline-regulated gene expression or gene disruption) or destroying the mature mRNA (RNAi technology). In both cases, the target protein...

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Detalhes bibliográficos
Main Authors: Laura Wood, Daniel G. Booth, Giulia Vargiu, Shinya Ohta, Flavia deLima Alves, Kumiko Samejima, Tatsuo Fukagawa, Juri Rappsilber, William C. Earnshaw
Formato: Artigo
Idioma:Inglês
Publicado em: The Royal Society 2016-01-01
Colecção:Open Biology
Assuntos:
kinetochore
cenp-t/w
chromosomes
aid system
silac
mass spectrometry
Acesso em linha:https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.150230
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