Investigation of anomalous charge variant profile reveals discrete pH-dependent conformations and conformation-dependent charge states within the CDR3 loop of a therapeutic mAb

During the development of a therapeutic monoclonal antibody (mAb-1), the charge variant profile obtained by pH-gradient cation exchange chromatography (CEX) contained two main peaks, each of which exhibited a unique intrinsic fluorescence profile and demonstrated inter-convertibility upon reinjectio...

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Main Authors: Wenkui Lan, Joseph J. Valente, Andrew Ilott, Naresh Chennamsetty, Zhihua Liu, Joseph M. Rizzo, Aaron P. Yamniuk, Difei Qiu, Holly M. Shackman, Mark S. Bolgar
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis Group 2020-01-01
Colecção:mAbs
Assuntos:
pH-dependent conformation
charge heterogeneity
conformational equilibrium
HC-CDR3
therapeutic monoclonal antibody
cation exchange chromatography
Acesso em linha:https://www.tandfonline.com/doi/10.1080/19420862.2020.1763138
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