Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity

CRISPR-Cas9 has been widely used to manipulate genomes, however control over activity is necessary to explore transcriptional or epigenetic regulation. Here the authors use a split Cas9 fused to nuclear receptor ligand-binding domains to achieve tuneable Cas9 activity.

Bibliographic Details

Main Authors:	Duy P. Nguyen, Yuichiro Miyaoka, Luke A. Gilbert, Steven J. Mayerl, Brian H. Lee, Jonathan S. Weissman, Bruce R. Conklin, James A. Wells
Format:	Artigo
Language:	Inglês
Published:	Nature Portfolio 2016-07-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/ncomms12009
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