Localization of Disulfide Bonds in Ribonuclease Using Low pH Trypsin and LC-ESI-QTOF-MS

We evaluated a method to localize disulfide bonds in bovine pancreatic ribonuclease (RNase) by applying low pH trypsin protein digestion with a bottom-up LC-ESI-QTOF-MS approach. The goal was to minimize disulfide bond scrambling during sample preparation. By using N-ethylmaleimide (NEM) for alkylat...

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Prif Awduron:	Amélie Bornex, Saša M. Miladinović
Fformat:	Artigo
Iaith:	Alemão
Cyhoeddwyd:	Swiss Chemical Society 2024-12-01
Cyfres:	CHIMIA
Pynciau:	Bottom-up proteomics Disulphide bonds LC-MS Low pH trypsin
Mynediad Ar-lein:	https://www.chimia.ch/chimia/article/view/7475
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