Localization of Disulfide Bonds in Ribonuclease Using Low pH Trypsin and LC-ESI-QTOF-MS
We evaluated a method to localize disulfide bonds in bovine pancreatic ribonuclease (RNase) by applying low pH trypsin protein digestion with a bottom-up LC-ESI-QTOF-MS approach. The goal was to minimize disulfide bond scrambling during sample preparation. By using N-ethylmaleimide (NEM) for alkylat...
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| Principais autores: | , |
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| Formato: | Artigo |
| Idioma: | Alemão |
| Publicado em: |
Swiss Chemical Society
2024-12-01
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| coleção: | CHIMIA |
| Assuntos: | |
| Acesso em linha: | https://www.chimia.ch/chimia/article/view/7475 |
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