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Localization of Disulfide Bonds in Ribonuclease Using Low pH Trypsin and LC-ESI-QTOF-MS

We evaluated a method to localize disulfide bonds in bovine pancreatic ribonuclease (RNase) by applying low pH trypsin protein digestion with a bottom-up LC-ESI-QTOF-MS approach. The goal was to minimize disulfide bond scrambling during sample preparation. By using N-ethylmaleimide (NEM) for alkylat...

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Principais autores: Amélie Bornex, Saša M. Miladinović
Format: Artigo
Sprog:Alemão
Udgivet: Swiss Chemical Society 2024-12-01
Serier:CHIMIA
Fag:
Bottom-up proteomics
Disulphide bonds
LC-MS
Low pH trypsin
Online adgang:https://www.chimia.ch/chimia/article/view/7475
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