Improved yield and accuracy for DNA extraction in microbiome studies with variation in microbial biomass

A major challenge for microbiome studies is maintaining an even and accurate DNA extraction in the presence of samples with a wide range of bacterial content. Here we compare five DNA extraction methods using replicate stool samples that were diluted to create high and low biomass samples. Our resul...

Бүрэн тодорхойлолт

Номзүйн дэлгэрэнгүй

Үндсэн зохиолчид:	Amy Davis, Christina Kohler, Ramzi Alsallaq, Randall Hayden, Gabriela Maron, Elisa Margolis
Формат:	Artigo
Хэл сонгох:	Inglês
Хэвлэсэн:	Taylor & Francis Group 2019-06-01
Цуврал:	BioTechniques
Нөхцлүүд:	bacterial load DNA extraction high throughput low bacterial load metagenomics microbiome
Онлайн хандалт:	https://www.future-science.com/doi/10.2144/btn-2019-0016
Шошгууд:	Шошго нэмэх Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!