Improved yield and accuracy for DNA extraction in microbiome studies with variation in microbial biomass

A major challenge for microbiome studies is maintaining an even and accurate DNA extraction in the presence of samples with a wide range of bacterial content. Here we compare five DNA extraction methods using replicate stool samples that were diluted to create high and low biomass samples. Our resul...

Täydet tiedot

Bibliografiset tiedot

Päätekijät:	Amy Davis, Christina Kohler, Ramzi Alsallaq, Randall Hayden, Gabriela Maron, Elisa Margolis
Aineistotyyppi:	Artigo
Kieli:	Inglês
Julkaistu:	Taylor & Francis Group 2019-06-01
Sarja:	BioTechniques
Aiheet:	bacterial load DNA extraction high throughput low bacterial load metagenomics microbiome
Linkit:	https://www.future-science.com/doi/10.2144/btn-2019-0016
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