Improved yield and accuracy for DNA extraction in microbiome studies with variation in microbial biomass

A major challenge for microbiome studies is maintaining an even and accurate DNA extraction in the presence of samples with a wide range of bacterial content. Here we compare five DNA extraction methods using replicate stool samples that were diluted to create high and low biomass samples. Our resul...

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Detalhes bibliográficos

Principais autores:	Amy Davis, Christina Kohler, Ramzi Alsallaq, Randall Hayden, Gabriela Maron, Elisa Margolis
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Taylor & Francis Group 2019-06-01
coleção:	BioTechniques
Assuntos:	bacterial load DNA extraction high throughput low bacterial load metagenomics microbiome
Acesso em linha:	https://www.future-science.com/doi/10.2144/btn-2019-0016
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