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Improved yield and accuracy for DNA extraction in microbiome studies with variation in microbial biomass

A major challenge for microbiome studies is maintaining an even and accurate DNA extraction in the presence of samples with a wide range of bacterial content. Here we compare five DNA extraction methods using replicate stool samples that were diluted to create high and low biomass samples. Our resul...

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Wedi'i Gadw mewn:
Prif Awduron: Amy Davis, Christina Kohler, Ramzi Alsallaq, Randall Hayden, Gabriela Maron, Elisa Margolis
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Taylor & Francis Group 2019-06-01
Cyfres:BioTechniques
Pynciau:
bacterial load
DNA extraction
high throughput
low bacterial load
metagenomics
microbiome
Mynediad Ar-lein:https://www.future-science.com/doi/10.2144/btn-2019-0016
Tagiau: Ychwanegu Tag
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