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Targeted insertion of conditional expression cassettes into the mouse genome using the modified i-PITT

Abstract Background Transgenic (Tg) mice are widely used in biomedical research, and they are typically generated by injecting transgenic DNA cassettes into pronuclei of one-cell stage zygotes. Such animals often show unreliable expression of the transgenic DNA, one of the major reasons for which is...

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I tiakina i:
Ngā taipitopito rārangi puna kōrero
Ngā kaituhi matua: Hiromi Miura, Ayaka Nakamura, Aki Kurosaki, Ai Kotani, Masaru Motojima, Keiko Tanaka, Shigeru Kakuta, Sanae Ogiwara, Yuhsuke Ohmi, Hirotaka Komaba, Samantha L.P. Schilit, Cynthia C. Morton, Channabasavaiah B. Gurumurthy, Masato Ohtsuka
Hōputu: Artigo
Reo:Inglês
I whakaputaina: BMC 2024-06-01
Rangatū:BMC Genomics
Ngā marau:
Urunga tuihono:https://doi.org/10.1186/s12864-024-10250-0
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