Targeted insertion of conditional expression cassettes into the mouse genome using the modified i-PITT

Abstract Background Transgenic (Tg) mice are widely used in biomedical research, and they are typically generated by injecting transgenic DNA cassettes into pronuclei of one-cell stage zygotes. Such animals often show unreliable expression of the transgenic DNA, one of the major reasons for which is...

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التفاصيل البيبلوغرافية

المؤلفون الرئيسيون:	Hiromi Miura, Ayaka Nakamura, Aki Kurosaki, Ai Kotani, Masaru Motojima, Keiko Tanaka, Shigeru Kakuta, Sanae Ogiwara, Yuhsuke Ohmi, Hirotaka Komaba, Samantha L.P. Schilit, Cynthia C. Morton, Channabasavaiah B. Gurumurthy, Masato Ohtsuka
التنسيق:	Artigo
اللغة:	Inglês
منشور في:	BMC 2024-06-01
سلاسل:	BMC Genomics
الموضوعات:	Targeted transgenesis Conditional expression PhiC31 integrase FLP-FRT Pronuclear injection-based targeted transgenesis Mouse
الوصول للمادة أونلاين:	https://doi.org/10.1186/s12864-024-10250-0
الوسوم:	إضافة وسم لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!