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Targeted insertion of conditional expression cassettes into the mouse genome using the modified i-PITT

Abstract Background Transgenic (Tg) mice are widely used in biomedical research, and they are typically generated by injecting transgenic DNA cassettes into pronuclei of one-cell stage zygotes. Such animals often show unreliable expression of the transgenic DNA, one of the major reasons for which is...

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Principais autores: Hiromi Miura, Ayaka Nakamura, Aki Kurosaki, Ai Kotani, Masaru Motojima, Keiko Tanaka, Shigeru Kakuta, Sanae Ogiwara, Yuhsuke Ohmi, Hirotaka Komaba, Samantha L.P. Schilit, Cynthia C. Morton, Channabasavaiah B. Gurumurthy, Masato Ohtsuka
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2024-06-01
Serier:BMC Genomics
Fag:
Targeted transgenesis
Conditional expression
PhiC31 integrase
FLP-FRT
Pronuclear injection-based targeted transgenesis
Mouse
Online adgang:https://doi.org/10.1186/s12864-024-10250-0
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