ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes

ABSTRACT Two efficient recombination systems were combined to produce a versatile method for chromosomal engineering that obviates the need to prepare double-stranded DNA (dsDNA) recombination substrates. A synthetic “targeting oligonucleotide” is incorporated into the chromosome via homologous reco...

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Principais autores:	Kenan C. Murphy, Samantha J. Nelson, Subhalaxmi Nambi, Kadamba Papavinasasundaram, Christina E. Baer, Christopher M. Sassetti
格式:	Artigo
語言:	Inglês
出版:	American Society for Microbiology 2018-12-01
叢編:	mBio
主題:	Mycobacterium smegmatis bacteriophage genetics gene replacement genetic fusions metabolic engineering promoter replacements
在線閱讀:	https://journals.asm.org/doi/10.1128/mBio.01467-18
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