ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes
ABSTRACT Two efficient recombination systems were combined to produce a versatile method for chromosomal engineering that obviates the need to prepare double-stranded DNA (dsDNA) recombination substrates. A synthetic “targeting oligonucleotide” is incorporated into the chromosome via homologous reco...
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| Hlavní autoři: | , , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology
2018-12-01
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| Edice: | mBio |
| Témata: | |
| On-line přístup: | https://journals.asm.org/doi/10.1128/mBio.01467-18 |
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