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ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes

ABSTRACT Two efficient recombination systems were combined to produce a versatile method for chromosomal engineering that obviates the need to prepare double-stranded DNA (dsDNA) recombination substrates. A synthetic “targeting oligonucleotide” is incorporated into the chromosome via homologous reco...

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Xehetasun bibliografikoak
Egile Nagusiak: Kenan C. Murphy, Samantha J. Nelson, Subhalaxmi Nambi, Kadamba Papavinasasundaram, Christina E. Baer, Christopher M. Sassetti
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: American Society for Microbiology 2018-12-01
Saila:mBio
Gaiak:
Sarrera elektronikoa:https://journals.asm.org/doi/10.1128/mBio.01467-18
Etiketak: Etiketa erantsi
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