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ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes

ABSTRACT Two efficient recombination systems were combined to produce a versatile method for chromosomal engineering that obviates the need to prepare double-stranded DNA (dsDNA) recombination substrates. A synthetic “targeting oligonucleotide” is incorporated into the chromosome via homologous reco...

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Autori principali: Kenan C. Murphy, Samantha J. Nelson, Subhalaxmi Nambi, Kadamba Papavinasasundaram, Christina E. Baer, Christopher M. Sassetti
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2018-12-01
Serie:mBio
Soggetti:
Mycobacterium smegmatis
bacteriophage genetics
gene replacement
genetic fusions
metabolic engineering
promoter replacements
Accesso online:https://journals.asm.org/doi/10.1128/mBio.01467-18
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