Analysis of Protein Stability by Synthesis Shutoff
In this protocol, we describe the analysis of protein stability over time, using synthesis shutoff. As an example, we express HA-tagged yeast mitofusin Fzo1 in Saccharomyces cerevisiae and inhibit translation via cycloheximide (CHX). Proteasomal inhibition with MG132 is performed, as an optional ste...
Sparad:
| Huvudupphov: | , , |
|---|---|
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Utgiven: |
Bio-protocol LLC
2021-11-01
|
| Serie: | Bio-Protocol |
| Länkar: | https://bio-protocol.org/en/bpdetail?id=4225&type=0 |
| Taggar: |
Inga taggar, Lägg till första taggen!
|