Analysis of Protein Stability by Synthesis Shutoff
In this protocol, we describe the analysis of protein stability over time, using synthesis shutoff. As an example, we express HA-tagged yeast mitofusin Fzo1 in Saccharomyces cerevisiae and inhibit translation via cycloheximide (CHX). Proteasomal inhibition with MG132 is performed, as an optional ste...
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| Autori principali: | , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Bio-protocol LLC
2021-11-01
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| Serie: | Bio-Protocol |
| Accesso online: | https://bio-protocol.org/en/bpdetail?id=4225&type=0 |
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