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Polyelectrolyte Surface Interface for Single-Molecule Fluorescence Studies of DNA Polymerase

We report the use of polyelectrolyte multilayers in a stable robust surface chemistry for specific anchoring of DNA to glass. The nonspecific binding of fluorescently tagged nucleotides is suppressed down to the single-molecule level, and DNA polymerase is active on the anchored DNA template. This s...

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Bibliografiske detaljer
Principais autores: Emil P. Kartalov, Marc A. Unger, Stephen R. Quake
Format: Artigo
Sprog:Inglês
Udgivet: Taylor & Francis Group 2003-03-01
Serier:BioTechniques
Online adgang:https://www.future-science.com/doi/10.2144/03343st02
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