Polyelectrolyte Surface Interface for Single-Molecule Fluorescence Studies of DNA Polymerase
We report the use of polyelectrolyte multilayers in a stable robust surface chemistry for specific anchoring of DNA to glass. The nonspecific binding of fluorescently tagged nucleotides is suppressed down to the single-molecule level, and DNA polymerase is active on the anchored DNA template. This s...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Taylor & Francis Group
2003-03-01
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| Schriftenreihe: | BioTechniques |
| Online-Zugang: | https://www.future-science.com/doi/10.2144/03343st02 |
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